Description

Mass spectrometry (MS) is an analytical technique that is used to identify unknown compounds, quantify known compounds, and elucidate the structure and chemical properties of molecules. Originally used by pharmaceutical companies to conduct drug research, MS has a host of potential applications-ranging from detecting chemical agents for biodefense to facilitating the quantification of complex proteins. Traditionally large, intricate and expensive instruments, mass spectrometers are most often found in core facilities, however, lower cost models have recently been launched-enabling smaller groups of life scientists to acquire them. As more scientists gain access to these instruments and the field of mass spectrometry evolves, mass spectrometer suppliers must understand the needs of the market in order to improve their instruments and make them more powerful. Designed to provide a detailed analysis of the MS market from the end-user's perspective, Mass Spectrometry: Opportunities in the Life Science Market is based on 35-question survey of 850 scientists who currently use, or plan to use, MS in their research. The report provides a comprehensive look at researchers' experimental design, including current and future throughput, sample preparation and instrument configurations used. By identifying the rate at which new researchers are entering the field as well as scientists' current and future applications of MS and their anticipated purchasing plans, this report will help suppliers better understand the direction of the market and its projected growth. One application where the use of MS is increasing is in protein quantification. To provide a closer look at the potential in quantitative proteomics, the report specifically explores the use of, and satisfaction with, various methods used to quantify proteins and the developments needed to encourage widespread use of this exciting technology. The report also addresses the competitive dynamics of the market and identifies the suppliers from which researchers are most likely to purchase their next mass spectrometer. It thoroughly examines scientists' loyalty to the supplier of their most recently purchased mass spectrometer as well as purchasing motivations, likelihood to recommend that supplier and likelihood to re-purchase. Additionally, it assesses users' satisfaction with specific features of the instrument. By using this report to understand the actual experiences, preferences and expectations of researchers, mass spectrometer suppliers can better position themselves and appeal to the diverse needs of their target market. A combination of enhancements and innovations will extend the use of mass spectrometry into additional laboratories and toward novel applications. Mass Spectrometry: Opportunities in the Life Science Market contains over 60 charts and/or tables and more than 20 cross-tabulations for the 35 survey questions. Based on question 1, 653 respondents who currently conduct use MS were directed to answer most questions, while 197 respondents who plan to use MS within the next 12 months only answered Questions 2, 4, 5, 31 and 36. Below is a glimpse of the key findings derived from just a few of the survey questions: • The scientists surveyed who either currently, or plan to, use MS in their research best describe themselves as biochemists (33%) or molecular biologists (32%). • More than half of the respondents indicated that the mass spectrometer they use is not in a core/service lab. • Of the scientists who plan to purchase a mass spectrometer, 45 percent plan to do so within the next 6 months and the majority are most likely to purchase from Applied Biosystems and Thermo Electron • Greater sensitivity was the reason one-quarter of the respondents purchased their newest mass spectrometer from the supplier they did. • The scientists who indicated that they currently use MS to quantify protein expression cited most frequently using 2-D PAGE (not DiGE) and tandem MS (MS/MS) to do so. (electronic copy includes one print copy)

Table of Contents

Section 1. Analysis and Interpretation of Survey Results Introduction Application-Driven Mass Spectrometer Parameters 1-3 Ionization Techniques 1-4 Instrument Configurations 1-6 Sample Preparation and Complexity 1-7 Throughput Levels 1-8 Sample Origin Mass Spec Market Dynamics 1-9 Leading Suppliers Customer Satisfaction and Loyalty 1-11 Product Performance Attributes 1-12 Overall Satisfaction by Brand 1-13 Likelihood to Repurchase and/or Recommend 1-15 Customer Loyalty Scores by Brand Market Dynamics 1-16 Influencers of Future Purchasing 1-17 Purchase Motivation 1-18 Expectations for Growth Emerging Applications: Focus on Quantitative Proteomics 1-19 Quantitative Proteomics Use and Methods 1-20 Product Performance Attributes 1-21 Desired Improvements Conclusion Section 2. Study Methodology and Demographics 2-1 Methodology 2-6 Definition of Terms 2-7 Demographics 2-8 Questionnaire Section 3. Presentation of Survey Data Usage and Applications (Respondents = 653) 3-1 Use of mass spectrometry in research 3-5 Types of scientists* 3-6 Applications for which mass spectrometry is performed 3-7 Applications for which there are plans to perform mass spectrometry* 3-8 Origins of the primary sample studied using mass spectrometry* 3-11 Most significant advantages of identifying proteins using mass spectrometry 3-12 Ways in which protein samples are prepared for mass spectrometer analysis 3-13 Complexity of the samples analyzed by mass spectrometry 3-14 Locations of the mass spectrometers used most often 3-15 Future plans to purchase mass spectrometers 3-16 Timeframe for purchasing new mass spectrometers** 3-17 Suppliers from which new mass spectrometers are most likely to be purchased** Throughput (Respondents = 380) 3-19 Average number of mass spectrometer analyses performed per month 3-20 Change in number of analyses performed over the next 12 months Mass Spectrometer Instruments (Respondents = 380) 3-22 Number of accessible mass spectrometers 3-23 Suppliers of accessible mass spectrometers and suppliers of the newest mass spectrometers 3-25 Ionization sources used in mass spectrometry 3-26 Ion detectors used in mass spectrometry 3-27 Instrument configurations used in mass spectrometry Most Recently Purchased Mass Spectrometers (Respondents = 380) 3-28 When the newest mass spectrometers were purchased 3-29 Reasons why mass spectrometers were purchased from particular suppliers 3-30 Types of software used to analyze the data obtained from the most recently purchased mass spectrometers 3-31 Types of information provided by the software used to analyze the spectral data from the most recently purchased mass spectrometers 3-32 Satisfaction with specific features of the most recently purchased mass spectrometers 3-34 Overall satisfaction with the most recently purchased mass spectrometers 3-35 Change in opinion of most recent supplier 3-36 Likelihood to purchase a mass spectrometer again from the same suppliers 3-37 Likelihood to purchase other equipment from the most recent suppliers 3-38 Likelihood to recommend a mass spectrometer from the most recent suppliers 3-39 Most important features of a new mass spectrometer Quantitative Proteomics (Respondents = 99) 3-40 Familiarity with methods to quantify protein expression 3-43 Planned use of methods to quantify protein expression 3-44 Satisfaction with specific characteristics of the method used for protein quantification 3-46 Overall satisfaction with the method used for protein quantification 3-47 Performance of multiplexing 3-48 Most important areas of development to increase the widespread use of quantitative proteomics 3-4 Greatest challenges with regards to the adoption of mass spectrometry Section 4. Appendices 4-1 Insights and Perspectives 4-27 Cross-Tabulations of Survey Data 4-112 Quadrant Analysis for Mass Spectrometer Attributes 4-119 Other Recent Publications 4-121 About BioInformatics 4-122 Our Valued Clients